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Update Cluster_v2.0.py
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f6c34517ae
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@ -25,10 +25,10 @@ def input_validation(value, error_message):
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print(error_message)
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exit(1)
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def cluster_sequences(program, threshold, overlap, input_folder, output_folder):
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def cluster_sequences(program, identity, overlap, input_folder, output_folder):
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for file in tqdm(os.listdir(input_folder)):
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if file.endswith('.fasta'):
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subprocess.run([f'{program}', '-i', f'{input_folder}/{file}', '-o', f'{output_folder}/{file}', '-c', f'{threshold}', '-d', '0', '-aS', f'{overlap}'])
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subprocess.run([f'{program}', '-i', f'{input_folder}/{file}', '-o', f'{output_folder}/{file}', '-c', f'{identity}', '-d', '0', '-aS', f'{overlap}'])
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for file in os.listdir(output_folder):
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if file.endswith('.clstr'):
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@ -52,13 +52,13 @@ def main():
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os.mkdir(args.output)
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if args.type == 'aa':
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threshold = input_validation(args.identity, 'ERROR! Use format 0.## for Amino acids sequence identity threshold.')
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identity = input_validation(args.identity, 'ERROR! Use format 0.## for Amino acids sequence identity threshold.')
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overlap = input_validation(args.overlap, 'ERROR! Use format 0.## for Amino acids sequence alignment overlap value.')
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cluster_sequences('cd-hit', threshold, overlap, args.input, args.output)
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cluster_sequences('cd-hit', identity, overlap, args.input, args.output)
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elif args.type == 'dna':
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threshold = input_validation(args.identity, 'ERROR! Use format 0.## for DNA sequence identity threshold.')
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identity = input_validation(args.identity, 'ERROR! Use format 0.## for DNA sequence identity threshold.')
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overlap = input_validation(args.overlap, 'ERROR! Use format 0.## for DNA sequence alignment overlap value.')
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cluster_sequences('cd-hit-est', threshold, overlap, args.input, args.output)
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cluster_sequences('cd-hit-est', identity, overlap, args.input, args.output)
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else:
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print('Invalid sequence type. Choose "aa" for Amino Acids or "dna" for DNA.')
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exit(1)
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