diff --git a/Utilities/for_fastas/CUB.py b/Utilities/for_fastas/CUB.py
index 3de1877..d080441 100644
--- a/Utilities/for_fastas/CUB.py
+++ b/Utilities/for_fastas/CUB.py
@@ -4,7 +4,8 @@
 #Dependencies: Python3, numpy, BioPython
 #Inputs: Fasta file or folder of fasta files
 #Outputs: A fasta file filtered for properly formatted sequences and several spreadsheets summarizing GC, ENc, RSCU, etc.
-#Example: python3 CUB.py -i seqs.fasta
+#Example: python3 CUB.py -i seqs.fasta 
+#Note: Use "python3 CUB.py -i seqs.fasta --require_start --require_stop" when using the on R2G files.
 
 #Dependencies
 import os