import os,sys with open('ScoreBySeq.tsv', 'w') as o: for clade in os.listdir('GuidanceOutput'): if(os.path.isfile('GuidanceOutput/' + clade + '/MSA.MAFFT.Guidance2_res_pair_seq.scr_with_Names')): for line in open('GuidanceOutput/' + clade + '/MSA.MAFFT.Guidance2_res_pair_seq.scr_with_Names'): if('SEQUENCE_NAME' not in line): o.write(line)