diff --git a/PhyloToL-Part-1:-GF-assignment.md b/PhyloToL-Part-1:-GF-assignment.md index 614f9bd..caad48f 100644 --- a/PhyloToL-Part-1:-GF-assignment.md +++ b/PhyloToL-Part-1:-GF-assignment.md @@ -71,7 +71,7 @@ Replacing the PhyloToL Hook DB with a user-defined set of gene families is strai Role of each script -Running PhyloToL Part 1 on transcriptomes requires at least 3 items in your main directory: 1) A folder named Scripts and containing all [Scripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Transcriptomes/Scripts) from PhyloToL part 1 github, 2) a folder containing your assembled [transcripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Transcriptomes/TestData) (as described above), and 3) a folder containing the Databases with three subfolders(db_BvsE (how we ID likely-bacterial sequences), db_StopFreq (for stop codon assignment), and db_OG (The hook database as described above)). Default script starts with your assembled transcripts and produces ReadyToGo files (nucleotide and amino acid sequences) of each taxa, and a summary information of the sequences processed for those taxa. +Running PhyloToL Part 1 on transcriptomes requires at least 3 items in your main directory: 1) A folder named Scripts and containing all **[Scripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Transcriptomes/Scripts)** from PhyloToL part 1 github, 2) a folder containing your **assembled [transcripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Transcriptomes/TestData)** (as described above), and 3) a folder containing the **Databases** with three subfolders(db_BvsE (how we ID likely-bacterial sequences), db_StopFreq (for stop codon assignment), and db_OG (The hook database as described above)). Default script starts with your **assembled transcripts** and produces **ReadyToGo files** (nucleotide and amino acid sequences) of each taxa, and **summary information** of the sequences processed for those taxa. * To run the PhyloToL part 1 for processing transcriptomes, run: `python Scripts/wrapper.py --first_script 1 --last_script 7 --assembled_transcripts AssembledTranscripts --output . --genetic_code Gcode.txt --databases Databases > log.txt` @@ -110,7 +110,7 @@ Example of a Conspecific.txt file Role of each script -Running PhyloToL Part 1 on genomes requires at least 3 items in your main directory: 1) A folder named **Scripts** and containing all [Scripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/Scripts) from PhyloToL part 1 github, 2) a folder containing your **[CDS](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/TestData)** (as described above), and 3) a folder containing the **Databases** with three subfolders(db_BvsE (how we ID likely-bacterial sequences), db_StopFreq (for stop codon assignment), and db_OG (The hook database as described above)). Default script starts with your **CDS** and produces **ReadyToGo files** (nucleotide and amino acid sequences) of each taxa, and **summary information** of the sequences processed for those taxa. +Running PhyloToL Part 1 on genomes requires at least 3 items in your main directory: 1) A folder named Scripts and containing all **[Scripts](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/Scripts)** from PhyloToL part 1 github, 2) a folder containing your **[CDS](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/TestData)** (as described above), and 3) a folder containing the **Databases** with three subfolders(db_BvsE (how we ID likely-bacterial sequences), db_StopFreq (for stop codon assignment), and db_OG (The hook database as described above)). Default script starts with your **CDS** and produces **ReadyToGo files** (nucleotide and amino acid sequences) of each taxa, and **summary information** of the sequences processed for those taxa. #### To run the PhyloToL part 1 for processing genomes, run: