diff --git a/EukPhylo-QuickStart.md b/EukPhylo-QuickStart.md
index 931aeb6..517163f 100644
--- a/EukPhylo-QuickStart.md
+++ b/EukPhylo-QuickStart.md
@@ -122,7 +122,7 @@ In a main project directory:
 
 Basic running for building MSAs and Trees:
 
-`python3 Scripts/phylotol.py --start raw --end trees --gf_list  OG_list.txt --taxon_list taxa.txt --data R2Gs --output Output  > Output.out`
+`python3 Scripts/phylotol.py --start raw --end trees --gf_list OG_list.txt --taxon_list taxa.txt --data R2Gs --output Output  > Output.out`
 
 For additional input parameter options, see table below or run: `python phylotol.py --help`