diff --git a/Home.md b/Home.md index cfcd67f..cedc8f0 100644 --- a/Home.md +++ b/Home.md @@ -1,4 +1,4 @@ -PhyloToL version 6 is designed to be a modular, accessible pipeline that includes sophisticated data curation methods, including a newly designed method of phylogeny-informed contamination removal, gene families homology estimation, and generating multisequence alignments (MSAs) and gene trees. +PhyloToL version 6 is designed to be a modular, accessible pipeline that includes sophisticated data curation methods, including a newly designed method of phylogeny-informed contamination removal, gene families homology estimation, and generating multisequence alignments (MSAs) and gene trees. More details can be found in the manuscript files here (**TBD insert BioRxiv link**) The core PhyloToL pipeline comprises two main components, which we refer to as PhyloToL parts 1 and 2. PhyloToL part 1 takes input sequences from a whole genome or transcriptome assembly, applies several curation steps, and provides initial homology assessment against a customizable database of reference sequences to assign GFs. Part one outputs a fasta file of curated nucleotide and amino acid sequences with gene families assigned, as well as a dataset of descriptive statistics (e.g. length, coverage, and composition) for each input sample. PhyloToL part 2 is highly modular; for a given selection of taxa and GFs it stringently assesses homology by iterating the tool Guidance (Penn et al., 2010; Sela et al., 2015), which outputs an MSA for each gene family. From MSAs it builds gene trees, and then includes an innovative workflow for tree topology-based contamination removal.