diff --git a/PhyloToL-Part-2:-MSAs,-trees,-and-contamination-loop.md b/PhyloToL-Part-2:-MSAs,-trees,-and-contamination-loop.md index 2edb5c4..8c7a5d7 100644 --- a/PhyloToL-Part-2:-MSAs,-trees,-and-contamination-loop.md +++ b/PhyloToL-Part-2:-MSAs,-trees,-and-contamination-loop.md @@ -1,6 +1,6 @@ # Overview and Modularity PhyloToL Part 2 is designed to: -1. Generate multisequence alignments (**MSAs**) firsts filtering in a "Pre-Guidance" step and then using [Guidance](https://taux.evolseq.net/guidance/source) version 2.02 , iterating to remove sequences considered non-homologs (based on sequence score is 0.3) +1. Generate multisequence alignments (**MSAs**) after first filtering in a "Pre-Guidance" step and then using [Guidance](https://taux.evolseq.net/guidance/source) version 2.02 , iterating to remove sequences considered non-homologs (based on sequence score is 0.3) 2. Generate gene trees using a 3rd party program (RaxML, IQTree, FastTree) 3. Remove contaminating sequences with the "Contamination Loop" (**CL**) and user-defined rules for both sister/subsister and clade grabbing options 4) Select orthologs and construct concatenated alignments for building species trees.