From 86754e89879bb67b18206728a985208b6ea1630e Mon Sep 17 00:00:00 2001 From: rebeccagawron <122105684+rebeccagawron@users.noreply.github.com> Date: Fri, 5 Jan 2024 11:26:58 -0500 Subject: [PATCH] Updated Set up (markdown) --- Set-up.md | 18 +++++++++++++++++- 1 file changed, 17 insertions(+), 1 deletion(-) diff --git a/Set-up.md b/Set-up.md index 042e1d0..d1a032b 100644 --- a/Set-up.md +++ b/Set-up.md @@ -1,5 +1,4 @@ ## Transcriptomes -* Assembled transcripts ### What you need in Transcriptomes folder: * A folder called “AssembledTranscripts” with your assembled transcript fasta files * A folder called “Databases” with the three folders: @@ -15,4 +14,21 @@ * NOTE that this step is only if you are running transcriptomes that all need specific genetic codes! * *A file called “Conspecific.txt” filled with your 10-digit codes in a column tab separated with the conspecific (e.g. genus, species or metatranscriptome) names for each sample +*not required for all PhyloToL runs + +## Genomes +### What you need in Genomes folder: +* A folder called “CDS” with your CDS files +* A folder called “Databases” with the three folders: + * db_BvsE (how we ID likely-bacterial sequences) + * db_StopFreq (for stop codon assignment) + * db_OG (this must be current version of the hook) + * Current version is Hook-6.6 (08/04/23) + * Files needed: + * Hook *.dmnd file ([Current version Hook-6.6.dmnd](https://drive.google.com/open?id=1ywYLZXzcTERDFCysz5vPbI9u6WRxz5r0&usp=drive_copy)) + * Hook *.fasta file ([Current version Hook-6.6.fasta](https://drive.google.com/open?id=1AN4_SmZUYFH6_xh2qOhyNUlFZ_NT9_-D&usp=drive_copy)) +* A folder called “Scripts” filled with the 10 scripts from [here on Github](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/Scripts) +* *A file called “Gcodes.txt” filled with your 10-digit codes in a column tab separated with the genetic code designation for each sample + * NOTE that this step is only if you are running genomes that all need specific genetic codes! + *not required for all PhyloToL runs \ No newline at end of file