diff --git a/PhyloToL-Part-2.md b/PhyloToL-Part-2-‐-MSAs,-CL,-Trees.md similarity index 89% rename from PhyloToL-Part-2.md rename to PhyloToL-Part-2-‐-MSAs,-CL,-Trees.md index 649dd00..e0c5b11 100644 --- a/PhyloToL-Part-2.md +++ b/PhyloToL-Part-2-‐-MSAs,-CL,-Trees.md @@ -1,6 +1,8 @@ # Overview and Modularity - +PhyloToL Part 2 is designed to: 1) generate multisequence alignments (**MSAs**) using Guidance (ref/github link ******), iterating to remove sequences that are not homologous (default sequence score is ****); 2) generate gene trees using a 3rd party program (RaxML, IQTree, FastTree); 3) remove contaminating sequences with the "Contamination Loop" (**CL**) and user-defined rules for both sister/subsister and clade grabbing options (see BioRxiv); and 4) ortholog selection and generation of species trees from concatenated alignments. + # Set Up +Requirements include: fasta files with controlled names. # Databases