diff --git a/EukPhylo-QuickStart.md b/EukPhylo-QuickStart.md
index 649423b..2ea9273 100644
--- a/EukPhylo-QuickStart.md
+++ b/EukPhylo-QuickStart.md
@@ -64,6 +64,7 @@ EukPhylo part 1 runs CDS (genomes) or assembled transcripts (transcriptomes) thr
         * Hook *.dmnd file ([Current version Hook-1.0.dmnd])
         * Hook *.fasta file ([Current version Hook-1.0.fasta]) 
 * A folder called “Scripts” with scripts from [here](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Transcriptomes/Scripts) on GitHub
+* An empty folder for the output named as indicated in the run command for flag -o
 
 ### Running:
 `