Updated PhyloToL Part 1 (markdown)

Katzlab 2024-08-09 17:26:26 -04:00
parent f8f9a1233e
commit dc4c401ad9

@ -54,6 +54,24 @@ And all of the CDS fasta files should be in a folder alongside the [Scripts](htt
## The Hook Database
PhyloToL6 is designed to have an interchangeable hook. As the Hook database is replaceable and customizable, this step offers an opportunity to filter data for a group of gene families/functional groups of interest.
###Swapping out the hook
What to know about using your own hook:
*
The hook lives in the Databases folder, specifically the db_OG folder
*
The fasta file contains …..
The diamond file contains …..
It is recommended that you create your own diamond database using your fasta file, so you can be sure that diamond will be compatible with your computers version
To do this, do the following command
diamond makedb --in <path to fasta file> -d <name you want to give it>.dmnd
Be aware that you must give your fasta file and your diamond file the same exact name, or else your run will fail at the end
For example, the Katzlab Hook-6.6 files are named:
Hook-6.6.fasta
Hook-6.6.dmnd
## Processing transcriptomes