#PBS -N 4_Sacc_cerevisiae_dec_cov1_ymj
#PBS -l nodes=1:ppn=48
#PBS -r y
#PBS -q master_431

#转录组不需要做这一步，直接跳到下一步

cd /home/Data_01/yizhenzhen06/public_share/Saccharomyces_cerevisiae_DNA/3_decontamination/

echo Start Time is `date`

blastn -db /home/Data_01/share_databases/human_genome_new/GCF_000001405.39_GRCh38.p13_rna_db \
-query 2_Sacc_cerevisiae_dec_bac_hum.fasta \
-out 3_Sacc_cerevisiae_dec_bac_hum_geno_rna.tab \
-outfmt "6 qseqid qlen sseqid slen pident qcovs qstart qend sstart send mismatch gapopen evalue bitscore" \
-evalue 1e-5 -max_target_seqs 1 -num_threads 48

sed -i '1i\tquery ID\tquery sequence length\tsubject ID\tsubject qequence length\tpercent identities\tcoverage\tqstart\tqend\tsstart\tsend\tmismatch\tgapopen\tevalue\tbitscores ' 3_Sacc_cerevisiae_dec_bac_hum_geno_rna.tab

echo End Time is `date`