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Updated Set up (markdown)

rebeccagawron 2024-01-05 11:26:58 -05:00
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Set-up.md

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## Transcriptomes
* Assembled transcripts
### What you need in Transcriptomes folder:
* A folder called “AssembledTranscripts” with your assembled transcript fasta files
* A folder called “Databases” with the three folders:
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* NOTE that this step is only if you are running transcriptomes that all need specific genetic codes!
* *A file called “Conspecific.txt” filled with your 10-digit codes in a column tab separated with the conspecific (e.g. genus, species or metatranscriptome) names for each sample
*not required for all PhyloToL runs
## Genomes
### What you need in Genomes folder:
* A folder called “CDS” with your CDS files
* A folder called “Databases” with the three folders:
* db_BvsE (how we ID likely-bacterial sequences)
* db_StopFreq (for stop codon assignment)
* db_OG (this must be current version of the hook)
* Current version is Hook-6.6 (08/04/23)
* Files needed:
* Hook *.dmnd file ([Current version Hook-6.6.dmnd](https://drive.google.com/open?id=1ywYLZXzcTERDFCysz5vPbI9u6WRxz5r0&usp=drive_copy))
* Hook *.fasta file ([Current version Hook-6.6.fasta](https://drive.google.com/open?id=1AN4_SmZUYFH6_xh2qOhyNUlFZ_NT9_-D&usp=drive_copy))
* A folder called “Scripts” filled with the 10 scripts from [here on Github](https://github.com/Katzlab/PhyloToL-6/tree/main/PTL1/Genomes/Scripts)
* *A file called “Gcodes.txt” filled with your 10-digit codes in a column tab separated with the genetic code designation for each sample
* NOTE that this step is only if you are running genomes that all need specific genetic codes!
*not required for all PhyloToL runs